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v on W illebrand factor, clotting factors, and clotting inhibitors in apheresis platelet concentrates
Author(s) -
Weiss Dominik R.,
Franke D.,
Strasser Erwin F.,
Ringwald Juergen,
Zimmermann Robert,
Eckstein Reinhold
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12304
Subject(s) - apheresis , clotting factor , platelet , chemistry , medicine , immunology
Background Apheresis platelet concentrates ( APCs ) are usually stored in citrated plasma at 22°C. The stability of coagulation proteins— v on W illebrand factor (v WF ), clotting factors ( CFs ), and their inhibitors—has often been described in association with the storage of thawed plasma. However, fewer data are available regarding changes in APCs . Study Design and Methods We measured CF activities and inhibitors in APCs on the day of manufacture ( D ay 0) and on D ays 4, 5, and 7. v WF was determined by measuring vWF antigen (v WF : A g) and vWF ristocetin cofactor (v WF : RCo ) and by multimer analysis. Results Twenty‐one PCs obtained by plateletpheresis were studied. Major changes were observed for Factor ( F ) VIII (37% loss of activity within 4 days), FV (20% within 4 days), and protein S (76% within 4 days). All other CF activities remained higher than 80% over the 7 days. Fibrinogen and the inhibitors antithrombin and protein C remained quite stable. FXI , FXII , and FXIII actually increased during storage (8, 11, and 12% within 4 days). v WF : Ag increased during storage of APCs by 2% per day, with a relative loss of v WF : RCo and high‐molecular‐weight multimers. Conclusion Even after 7 days of storage at 22°C, the hemostatic potential of the plasma content in APCs was roughly preserved. The increase in FXII antigen indicates that this CF may also be stored in platelets; however, this has not yet been described.