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PAS ‐ G supports platelet reconstitution after cryopreservation in the absence of plasma
Author(s) -
Johnson Lacey,
Reid Samantha,
Tan Shereen,
Vidovic Diana,
Marks Denese C.
Publication year - 2013
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12084
Subject(s) - cryopreservation , platelet , chemistry , andrology , centrifugation , dimethyl sulfoxide , fresh frozen plasma , in vitro , buffy coat , blood preservation , chromatography , biochemistry , immunology , biology , medicine , embryo , organic chemistry , microbiology and biotechnology
Background Platelet ( PLT ) concentrates frozen in dimethyl sulfoxide ( DMSO ) can be stored for extended periods at −80° C . PLT s are frozen in a hyperconcentrated state to avoid postthaw washing and minimize residual DMSO . Consequently, PLT s require reconstitution upon thawing. Although plasma, saline, and PLT additive solutions ( PAS s) have been used to reconstitute frozen PLT s, a comparison to define an optimal solution for PLT recovery has not been performed. Study Design and Methods DMSO (5% final concentration) was added to buffy coat–derived PLT s, followed by centrifugation to concentrate and freezing at −80° C . Cryopreserved PLT s (n = 12 per group) were thawed at 37° C , reconstituted in a unit of thawed frozen plasma, SSP +, or PAS ‐ G . In vitro PLT quality was examined before freezing, immediately after thawing, and 6 and 24 hours after thawing. Results After thawing and reconstitution, PLT s in plasma and PAS ‐ G displayed similar recovery (69 and 73%, respectively), while PLT recovery in SSP + was lower (62%). All PLT s maintained an acceptable pH and metabolic activity during postthaw storage. Frozen PLT s were activated, although the extent differed depending on the reconstitution solution, with PLT s in PAS ‐ G retaining better aggregation responses than PLT s in plasma or SSP +. Conclusion Thawing cryopreserved PLT s in PAS ‐ G , without plasma supplementation, resulted in PLTs with similar recovery and in vitro quality indicators as those suspended in plasma. Importantly, using PAS ‐ G enables the PLT s to be ready for use significantly faster than when having to thaw frozen plasma, which may be beneficial in trauma situations.