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Proteomic analysis of platelets treated with gamma irradiation versus a commercial photochemical pathogen reduction technology
Author(s) -
Marrocco Cristina,
D'Alessandro Angelo,
Girelli Gabriella,
Zolla Lello
Publication year - 2013
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12060
Subject(s) - platelet , proteome , apheresis , oxidative stress , proteomics , chemistry , mass spectrometry , oxidative phosphorylation , irradiation , metabolism , food irradiation , biochemistry , chromatography , biophysics , immunology , biology , gene , physics , nuclear physics
Background Several strategies are currently being tested to reduce the risk of pathogen transmission associated with platelet ( PLT ) transfusion. Within the framework of the I talian P latelet T echnology A ssessment S tudy, we investigated the variations of the protein profiles (proteomics) of apheresis PLT concentrates ( PCs ) upon treatment with riboflavin and ultraviolet ( UV ) light ( M irasol; 6.24 J/ mL ; 280‐400 nm). Study Design and Methods Control, gamma‐irradiated, and M irasol‐treated apheresis PC s were assayed on D ays 1 and 5 of storage by means of gel‐based analytical approaches (two‐dimensional gel electrophoresis) and mass spectrometry–based identification of significant (p < 0.05 analysis of variance) differential proteins. Supernatants were then assayed for metabolism and oxidative stress‐related metabolites through multiple reaction monitoring mass spectrometry. Results Only a handful of modifications could be observed in the PLT proteome profiles in response to the M irasol treatment, which included proteins involved in oxidative stress responses, PLT metabolism, and activation. Results confirmed increased metabolic rate and oxidative stress in the supernatants of treated PLTs (both gamma irradiated and M irasol treated). Conclusion From this investigation, it emerges that, from a proteomics standpoint, gamma irradiation results in the acceleration of PLT storage lesions and the M irasol treatment only moderately exacerbates these phenomena.