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Dynamic lateral organization of opioid receptors (kappa, mu wt and mu N40D ) in the plasma membrane at the nanoscale level
Author(s) -
Rogacki Maciej K.,
Golfetto Ottavia,
Tobin Steven J.,
Li Tianyi,
Biswas Sunetra,
Jorand Raphael,
Zhang Huiying,
Radoi Vlad,
Ming Yu,
Svenningsson Per,
Ganjali Daniel,
Wakefield Devin L.,
Sideris Athanasios,
Small Alexander R.,
Terenius Lars,
JovanovićTalisman Tijana,
Vukojević Vladana
Publication year - 2018
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12582
Subject(s) - receptor , κ opioid receptor , biophysics , μ opioid receptor , g protein coupled receptor , opioid receptor , opioid , biology , population , microbiology and biotechnology , biochemistry , medicine , environmental health
Using quantitative methods with single‐molecule sensitivity, fluorescence correlation spectroscopy and photoactivated localization microscopy, we characterized the nanoscale lateral dynamics and spatial organization of opioid receptors: kappa, mu, and mu isoform N40D. Experimental results supported by Monte Carlo simulations indicate that lateral organization is receptor‐specific and dynamic. Receptors differed in nanodomain size, population density, and fraction of receptors residing outside of nanodomains. The lateral organization of each receptor type was differently affected by cholesterol depletion from the plasma membrane.