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Ikarugamycin: A Natural Product Inhibitor of Clathrin‐Mediated Endocytosis
Author(s) -
Elkin Sarah R.,
Oswald Nathaniel W.,
Reed Dana K.,
Mettlen Marcel,
MacMillan John B.,
Schmid Sandra L.
Publication year - 2016
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12425
Subject(s) - endocytosis , endocytic cycle , clathrin , biology , microbiology and biotechnology , cell culture , cell , biochemistry , genetics
Ikarugamycin ( IKA ) is a previously discovered antibiotic, which has been shown to inhibit the uptake of oxidized low‐density lipoproteins in macrophages. Furthermore, several groups have previously used IKA to inhibit clathrin‐mediated endocytosis ( CME ) in plant cell lines. However, detailed characterization of IKA has yet to be performed. Consequently, we performed biochemistry and microscopy experiments to further characterize the effects of IKA on CME . We show that IKA has an IC 50 of 2.7 μ m in H1299 cells and acutely inhibits CME , but not other endocytic pathways, in a panel of cell lines. Although long‐term incubation with IKA has cytotoxic effects, the short‐term inhibitory effects on CME are reversible. Thus, IKA can be a useful tool for probing routes of endocytic trafficking.