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Identification and Characterization of LIN ‐2( CASK ) as a Regulator of Kinesin‐3 UNC ‐104( KIF1A ) Motility and Clustering in Neurons
Author(s) -
Wu GongHer,
Muthaiyan Shanmugam Muniesh,
Bhan Prerana,
Huang YuHsin,
Wagner Oliver Ingvar
Publication year - 2016
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12413
Subject(s) - microbiology and biotechnology , biology , bimolecular fluorescence complementation , caenorhabditis elegans , kinesin , scaffold protein , colocalization , activator (genetics) , microtubule , biochemistry , yeast , signal transduction , gene
LIN ‐2 and SYD ‐2 are both active zone proteins that interact with UNC ‐104 on overlapping binding regions. LIN ‐2 interacts with the motor via its L27 and GUK domains, which also interact with the coiled coils and SAM domains of SYD ‐2. While the absence of SYD ‐2 leads to a reduction in axonal motor clustering, the absence of LIN ‐2 increases clustering. Both LIN ‐2 and SYD ‐2 positively regulate the velocity of UNC ‐104, however, only LIN ‐2 is able to efficiently elevate the motor's run lengths.