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A Genetic Screen Identifies a Critical Role for the WDR81‐WDR91 Complex in the Trafficking and Degradation of Tetherin
Author(s) -
Rapiteanu Radu,
Davis Luther J.,
Williamson James C.,
Timms Richard T.,
Paul Luzio J.,
Lehner Paul J.
Publication year - 2016
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12409
Subject(s) - tetherin , endosome , biology , microbiology and biotechnology , ubiquitin , cathepsin l , downregulation and upregulation , transport protein , cathepsin , gene , viral envelope , virology , virus , genetics , biochemistry , intracellular , enzyme
A genetic screen in human haploid cells identifies a critical role for the WDR81‐WDR91 complex in the degradation of tetherin – a potent viral restriction factor. Subsequent experiments show that this complex plays a general role in the degradation of internalized plasma membrane proteins as it is also required for EGFR degradation. Depletion of WDR81 leads to the appearance of markedly enlarged early and late endocytic vesicles and to impaired delivery of cargo to lysosomes.