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Lipid Droplets Form from Distinct Regions of the Cell in the Fission Yeast Schizosaccharomyces pombe
Author(s) -
Meyers Alex,
Rio Zuania P.,
Beaver Rachael A.,
Morris Ryan M.,
Weiskittel Taylor M.,
Alshibli Amany K.,
Mannik Jaana,
MorrellFalvey Jennifer,
Dalhaimer Paul
Publication year - 2016
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12394
Subject(s) - schizosaccharomyces pombe , lipid droplet , schizosaccharomyces , phosphatidylethanolamine , phosphatidic acid , endoplasmic reticulum , microbiology and biotechnology , biology , diacylglycerol kinase , nucleus , biogenesis , cytoplasm , lipid metabolism , yeast , biochemistry , biophysics , phosphatidylcholine , saccharomyces cerevisiae , phospholipid , protein kinase c , membrane , gene , phosphorylation
The authors show that lipid droplets form from different regions of fission yeast Schizosaccharomyces pombe cells based on the dominant neutral lipid of the nascent droplet. Droplets that are enriched in sterol esters form at the tips of polarized cells, whereas droplets that are enriched in triacylglycerols ( TAGs ) form around the nucleus. Elimination of TAGs completely abolishes lipid droplets, instead vesicle‐shaped BODIPY 493/503 structures are observed. Thus, TAG seems necessary for lipid droplet biogenesis in these yeast cells.