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A Toolbox for Rapid Quantitative Assessment of Chronological Lifespan and Survival in Saccharomyces cerevisiae
Author(s) -
Chadwick Sarah R.,
Pananos Athanasios D.,
Di Gregorio Sonja E.,
Park Anna E.,
EtedaliZadeh Parnian,
Duennwald Martin L.,
Lajoie Patrick
Publication year - 2016
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12391
Subject(s) - saccharomyces cerevisiae , yeast , biology , model organism , longevity , cls upper limits , computational biology , microbiology and biotechnology , biochemistry , genetics , gene , medicine , optometry
The yeast Saccharomyces cerevisiae provides an unmatched genetic platform to study the mechanisms underlying the aging process. We present a rapid and efficient quantitative assay to measure chronological aging in yeast through fluorescent labeling of dead cells in multiwell plates. We designed an open‐source software to rapidly quantify yeast survival under various conditions, including chronological aging. We employed our assays to study the role of different heat shock proteins in the extension of yeast chronological lifespan by caloric restriction.