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Dynamin‐Actin Cross Talk Contributes to Phagosome Formation and Closure
Author(s) -
MarieAnaïs Florence,
Mazzolini Julie,
Herit Floriane,
Niedergang Florence
Publication year - 2016
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12386
Subject(s) - dynamin , phagosome , microbiology and biotechnology , pseudopodia , biology , endocytosis , phagocytosis , actin , cytokinesis , endocytic cycle , biophysics , biochemistry , cell , cell division
Phagosome formation relies on profound reorganization of actin and membranes, but the mechanism of phagosome closure remains poorly understood. We used an original experimental setup to monitor phagosome formation and closure in three dimensions in living macrophages using Total Internal Reflection Fluorescence ( TIRF ) Microscopy. We reveal that a cross talk between actin and dynamin‐2 takes place for phagosome formation and closure, and that dynamin‐2 plays a critical role in the effective scission of phagosomes from the plasma membrane.