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Measuring Exocytosis Rate Using Corrected Fluorescence Recovery After Photoconversion
Author(s) -
Luo Nan,
Yan An,
Yang Zhenbiao
Publication year - 2016
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12380
Subject(s) - exocytosis , endocytosis , biology , microbiology and biotechnology , biophysics , extracellular , membrane , biochemistry , cell
An optical method is developed to measure the exocytosis rate of plasma membrane or extracellular matrix proteins. In this method, the protein‐of‐interest is tagged with a green‐to‐red photoconvertible fluorescent protein; after photoconverting a region‐of‐interest on the cell surface, exocytosis‐dependent and ‐independent trafficking events are tracked simultaneously for accurate determination of exocytosis rate.