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Readily Accessible Multiplane Microscopy: 3D Tracking the HIV ‐1 Genome in Living Cells
Author(s) -
Itano Michelle S.,
Bleck Marina,
Johnson Daniel S.,
Simon Sanford M.
Publication year - 2016
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12347
Subject(s) - biology , microbiology and biotechnology , nucleolus , cytoplasm , rna , genome , computational biology , cell nucleus , endosome , virology , genetics , gene , intracellular
The trafficking of human immunodeficiency virus (HIV)‐1 genomic RNAs from sites of replication in the nucleus, through the cytoplasm, to sites of assembly at the plasma membrane is critical for HIV‐1 viral replication. We present a broadly accessible microscopy method that captures multiple focal planes simultaneously, enabling high‐resolution 3D imaging of a single macromolecule. We show that HIV‐1 genomic RNAs are most mobile in the cytosol, and undergo confined mobility at sites along the nuclear envelope and in the nucleus and nucleolus.