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Dengue Virus Uses a Non‐Canonical Function of the Host GBF1 ‐Arf‐ COPI System for Capsid Protein Accumulation on Lipid Droplets
Author(s) -
Iglesias Nestor G.,
Mondotte Juan A.,
Byk Laura A.,
De Maio Federico A.,
Samsa Marcelo M.,
Alvarez Cecilia,
Gamarnik Andrea V.
Publication year - 2015
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12305
Subject(s) - biology , copi , capsid , virus like particle , microbiology and biotechnology , dengue virus , host (biology) , virology , lipid droplet , function (biology) , dengue fever , virus , golgi apparatus , biochemistry , endoplasmic reticulum , genetics , secretory pathway , recombinant dna , gene
The dengue virus capsid protein accumulates progressively around cellular organelles named lipid droplets ( LDs ) during infection. Using different methods of intervention, we defined that capsid transport from virally modified endoplasmic reticulum ( ER ) membranes to LDs requires a functional GBF1 protein. The process was found to be COPI dependent, while it was independent of both COPII components and Golgi integrity, supporting a non‐canonical function of GBF1 / ARF / COPI transport system.