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High‐Resolution Membrane Capacitance Measurements for Studying Endocytosis and Exocytosis in Yeast
Author(s) -
Carrillo Lucia,
Cucu Bayram,
Bandmann Vera,
Homann Ulrike,
Hertel Brigitte,
Hillmer Stefan,
Thiel Gerhard,
Bertl Adam
Publication year - 2015
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12275
Subject(s) - exocytosis , endocytosis , biology , microbiology and biotechnology , yeast , membrane , endosome , capacitance , vesicular transport proteins , biophysics , cell , biochemistry , intracellular , chemistry , electrode , vacuolar protein sorting
With patch‐clamp recording, we detect in yeast protoplasts individual exo‐ and endocytotic events as discrete steps in membrane capacitance. The high‐resolution data show that exo‐ and endocytotic vesicles undergo, similar to other eukaryotes, permanent and transient fusion/fission with a bias for transient events. The electrical data are a good representation of the membrane dynamics of a growing yeast cell.