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Molecular Analysis and Localization of CaARA7 a Conventional RAB5 GTPase from Characean Algae
Author(s) -
Hoepflinger Marion C.,
Geretschlaeger Anja,
Sommer Aniela,
Hoeftberger Margit,
Hametner Christina,
Ueda Takashi,
Foissner Ilse
Publication year - 2015
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12267
Subject(s) - gtpase , biology , microbiology and biotechnology , endosome , rab , transport protein , gtpase activating protein , gtp' , small gtpase , biochemistry , g protein , signal transduction , intracellular , enzyme
This is the first detailed investigation of a conventional RAB5 GTPase ( CaARA7/CaRABF2 ) in a green alga closely related to land plants ( Chara australis ; Charales). CaARA7 is highly similar to other RAB5 proteins and has intrinsic GTPase activity. It is involved in endosomal membrane trafficking as suggested by localization experiments (immunolabelling of internodal cells using anti‐ CaARA7 as well as transient expression of different fluorescently tagged RAB5s in tobacco) and by protein mutations in membrane anchoring and GTP binding sites.