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Mammalian CORVET Is Required for Fusion and Conversion of Distinct Early Endosome Subpopulations
Author(s) -
Perini Enrico D.,
Schaefer Ramona,
Stöter Martin,
Kalaidzidis Yannis,
Zerial Marino
Publication year - 2014
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12232
Subject(s) - endosome , biology , microbiology and biotechnology , fusion protein , vesicular transport proteins , lipid bilayer fusion , recombinant dna , genetics , gene , intracellular , membrane , vacuolar protein sorting
The class C c or e v acuole/ e ndosome t ethering ( CORVET ) complex regulates endosomal tethering and maturation in yeast, but has never been studied in mammals. The mammalian CORVET complex is shown to be an effector of Rab5, and it is localized on the endosomal subpopulations marked by APPL1 and EEA1 . Depletion of CORVET subunits fragments APPL1 ‐positive but EEA1 ‐negative endosomes, and enlarges EEA1 endosomes. Our results indicate that the roles of CORVET in fusion and conversion have distinct impacts on different endosomal subpopulations.