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Issue Cover (June 2015)
Publication year - 2015
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12217
Subject(s) - endocytic cycle , endocytosis , microtubule , biology , microbiology and biotechnology , cholera toxin , cytoplasm , dynein , endosome , clathrin , organelle , cell , biochemistry , intracellular
Cover legend: Cholera toxin is enriched in plasma membrane tubules formed by cytoplasmic dynein. COS‐7 cells were ATP depleted prior to labeling with Alexa555‐CTxB and imaged live at 37°C on a confocal microscope. ATP depletion inhibits endocytic scission, leading to the generation of microns long, nascent endocytic tubules that are continuous with the plasma membrane. These invaginations are microtubule‐dependent and form through persistent microtubule motor activity of cytoplasmic dynein providing a pulling force on the cell surface. Using this assay, Day et al. define a novel role for microtubule motors in tubulating the plasma membrane during clathrin‐independent endocytosis. See Day et al. Traffic 2015; 16(6):572–590. Read the full article on doi: 10.1111/tra.12269