Premium
Quantitative Analysis of Membrane Trafficking in Regulation of Cdc42 Polarity
Author(s) -
Watson Leah J.,
Rossi Guendalina,
Brennwald Patrick
Publication year - 2014
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12211
Subject(s) - vesicle , microbiology and biotechnology , secretory vesicle , polarity (international relations) , golgi apparatus , vesicle fusion , cdc42 , cell polarity , biology , secretory pathway , endocytosis , membrane , biophysics , biochemistry , cell , synaptic vesicle , actin , endoplasmic reticulum
Using cells engineered to accumulate large clusters of post‐Golgi vesicles within the cytosol, we developed a novel assay to quantitatively examine the molecular density of Cdc42 on the surface of secretory vesicles compared to that at the plasma membrane polarity cap. The results demonstrate the immediate effect of membrane traffic is to dilute, rather than reinforce, Cdc42 polarity at the cell surface. This suggests that exocytic trafficking acts as a negative regulator of Cdc42 polarity.