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The CryoCapsule : Simplifying Correlative Light to Electron Microscopy
Author(s) -
Heiligenstein Xavier,
Heiligenstein Jérôme,
Delevoye Cédric,
Hurbain Ilse,
Bardin Sabine,
PaulGilloteaux Perrine,
Sengmanivong Lucie,
Régnier Gilles,
Salamero Jean,
Antony Claude,
Raposo Graca
Publication year - 2014
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12164
Subject(s) - microscopy , biology , electron microscope , microbiology and biotechnology , live cell imaging , fluorescence microscope , organelle , correlative , light sheet fluorescence microscopy , biophysics , cell , fluorescence , scanning confocal electron microscopy , optics , biochemistry , linguistics , physics , philosophy
Correlating complementary multiple scale images of the same object is a straightforward means to decipher biological processes. Light microscopy and electron microscopy are the most commonly used imaging techniques, yet despite their complementarity, the experimental procedures available to correlate them are technically complex. We designed and manufactured a new device adapted to many biological specimens, the CryoCapsule , that simplifies the multiple sample preparation steps, which at present separate live cell fluorescence imaging from contextual high‐resolution electron microscopy, thus opening new strategies for full correlative light to electron microscopy. We tested the biological application of this highly optimized tool on three different specimens: the in vitro Xenopus laevis mitotic spindle, melanoma cells over‐expressing YFP ‐langerin sequestered in organized membranous subcellular organelles and a pigmented melanocytic cell in which the endosomal system was labeled with internalized fluorescent transferrin.