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Binding to Any ESCRT Can Mediate Ubiquitin‐Independent Cargo Sorting
Author(s) -
Mageswaran Shrawan Kumar,
Dixon Megan Gorringe,
Curtiss Matt,
Keener James P.,
Babst Markus
Publication year - 2014
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12135
Subject(s) - escrt , endosome , microbiology and biotechnology , ubiquitin , biology , protein targeting , transport protein , transmembrane protein , cytoplasm , vesicle , membrane protein , biochemistry , membrane , receptor , gene , intracellular
We constructed fusion proteins that bind to endosomes and interact with ESCRTs . The trafficking analysis of these proteins showed that binding to ESCRTs caused either recycling (mimicking ESCRT machinery) or mediated ubiquitin‐independent sorting into the MVB pathway (mimicking cargo). Furthermore, efficient MVB sorting of soluble proteins was observed.