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Global Analysis of Apicomplexan Protein S‐Acyl Transferases Reveals an Enzyme Essential for Invasion
Author(s) -
Frénal Karine,
Tay Chwen L.,
Mueller Christina,
Bushell Ellen S.,
Jia Yonggen,
Graindorge Arnault,
Billker Oliver,
Rayner Julian C.,
SoldatiFavre Dominique
Publication year - 2013
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12081
Subject(s) - rhoptry , biology , palmitoylation , microbiology and biotechnology , biogenesis , organelle , toxoplasma gondii , protein targeting , plasmodium falciparum , apicomplexa , proteome , membrane protein , biochemistry , genetics , cysteine , enzyme , gene , membrane , malaria , antibody , immunology
Synopsis Spiderweb‐like gelatine matrices generated by electrospinning are introduced as versatile substrate for culturing animal cell monolayers destined for cryofixation and EM. Thus, non‐disruptive sampling and transfer of native cells into high‐pressure freezing devices is possible within about 30 seconds. In addition to cryosection‐ and replica‐labelling, this aproach can be applied to various complementary microscopy and biochemical methods.