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Class III Phosphoinositide 3‐Kinase/VPS34 and Dynamin are Critical for Apical Endocytic Recycling
Author(s) -
Carpentier Sarah,
N'Kuli Francisca,
Grieco Giuseppina,
Van Der Smissen Patrick,
Janssens Virginie,
Emonard Hervé,
Bilanges Benoît,
Vanhaesebroeck Bart,
Gaide Chevronnay Héloïse P.,
Pierreux Christophe E.,
Tyteca Donatienne,
Courtoy Pierre J.
Publication year - 2013
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12079
Subject(s) - endosome , dynamin , endocytic cycle , endocytosis , microbiology and biotechnology , biology , ly294002 , wortmannin , gtpase , pi3k/akt/mtor pathway , intracellular , receptor , biochemistry , signal transduction
Synopsis Recycling is a limiting step for receptor‐mediated endocytosis. We here show that class III PI3K is the key isoform involved, based on differential pharmacological inhibition (substractive approach) and PI‐3P sequestration by overexpressed GFP‐(FYVE)x2 in cultured kidney epithelial cells, as well as conditional PI3K‐III/Vps34 KO in developing mice kidneys. Acute reversal of PI3K inhibition induced a spectacular burst of recycling tubules that recruited dynamin‐GFP and depended on its GTPase activity. This simple procedure may help further dissect recycling machineries.