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Long‐read genome assembly and genetic architecture of fruit shape in the bottle gourd
Author(s) -
Xu Pei,
Wang Ying,
Sun Fengshuo,
Wu Rongling,
Du Huilong,
Wang Yuhong,
Jiang Libo,
Wu Xiaohua,
Wu Xinyi,
Yang Liming,
Xing Nailin,
Hu Yaowen,
Wang Baogen,
Huang Yunping,
Tao Ye,
Gao Qiang,
Liang Chengzhi,
Li Yanwei,
Lu Zhongfu,
Li Guojing
Publication year - 2021
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.15358
Subject(s) - lagenaria , bottle gourd , biology , gourd , genome , contig , cucurbitaceae , quantitative trait locus , botany , horticulture , genetics , gene
SUMMARY The bottle gourd ( Lagenaria siceraria , Cucurbitaceae) is an important horticultural crop exhibiting tremendous diversity in fruit shape. The genetic architecture of fruit shape variation in this species remains unknown. We assembled a long‐read‐based, high‐quality reference genome (ZAAS_Lsic_2.0) with a contig N50 value over 390‐fold greater than the existing reference genomes. We then focused on dissection of fruit shape using a one‐step geometric morphometrics‐based functional mapping approach. We identified 11 quantitative trait loci (QTLs) responsible for fruit shape (fsQTLs), reconstructed their visible effects and revealed syntenic relationships of bottle gourd fsQTLs with 12 fsQTLs previously reported in cucumber, melon or watermelon. Homologs of several well‐known and newly identified fruit shape genes, including SUN , OFP , AP2 and auxin transporters, were comapped with bottle gourd QTLs.