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FVE promotes RNA‐directed DNA methylation by facilitating the association of RNA polymerase V with chromatin
Author(s) -
Zhou JinXing,
Du Ping,
Liu ZhangWei,
Feng Chao,
Cai XueWei,
He XinJian
Publication year - 2021
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.15302
Subject(s) - chromatin , biology , genetics , dna methylation , rna directed dna methylation , chromatin remodeling , histone , epigenetics , heterochromatin , dna , microbiology and biotechnology , gene , gene expression
Summary Association of RNA polymerase V (Pol V) with chromatin is a critical step for RNA‐ directed DNA methylation (RdDM) in plants. Although the methylated DNA‐binding proteins SUVH2 and SUVH9 and the chromatin remodeler‐containing complex DRD1‐DMS3‐RDM1 are known to be required for the association of Pol V with chromatin, the molecular mechanisms underlying the association of Pol V with different chromatin environments remain largely unknown. Here we found that SUVH9 interacts with FVE, a homolog of the mammalian retinoblastoma‐associated protein, which has been previously identified as a shared subunit of the histone deacetylase complex and the polycomb‐type histone H3K27 trimethyltransferase complex. We demonstrated that FVE facilitates the association of Pol V with chromatin and thus contributes to DNA methylation at a substantial subset of RdDM target loci. Compared with FVE‐independent RdDM target loci, FVE‐dependent RdDM target loci are more abundant in gene‐rich chromosome arms than in pericentromeric heterochromatin regions. This study contributes to our understanding of how the association of Pol V with chromatin is regulated in different chromatin environments.