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Suppression of a BAHD acyltransferase decreases p ‐coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis
Author(s) -
Mota Thatiane R.,
Souza Wagner R.,
Oliveira Dyoni M.,
Martins Polyana K.,
Sampaio Bruno L.,
Vinecky Felipe,
Ribeiro Ana P.,
Duarte Karoline E.,
Pacheco Thályta F.,
Monteiro Norberto de K. V.,
Campanha Raquel B.,
Marchiosi Rogério,
Vieira Davi S.,
Kobayashi Adilson K.,
Molinari Patrícia A. de O.,
FerrareseFilho Osvaldo,
Mitchell Rowan A. C.,
Molinari Hugo B. C.,
Santos Wanderley D.
Publication year - 2021
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.15046
Subject(s) - arabinoxylan , setaria viridis , acyltransferase , biomass (ecology) , lignin , chemistry , setaria , cell wall , hydrolysis , biochemistry , hydroxycinnamic acid , food science , biology , botany , agronomy , enzyme , antioxidant , weed
Summary Grass cell walls have hydroxycinnamic acids attached to arabinosyl residues of arabinoxylan (AX), and certain BAHD acyltransferases are involved in their addition. In this study, we characterized one of these BAHD genes in the cell wall of the model grass Setaria viridis . RNAi silenced lines of S. viridis ( SvBAHD05 ) presented a decrease of up to 42% of ester‐linked p‐ coumarate ( p CA) and 50% of p CA‐arabinofuranosyl, across three generations. Biomass from SvBAHD05 silenced plants exhibited up to 32% increase in biomass saccharification after acid pre‐treatment, with no change in total lignin. Molecular dynamics simulations suggested that SvBAHD05 is a p ‐coumaroyl coenzyme A transferase (PAT) mainly involved in the addition of p CA to the arabinofuranosyl residues of AX in Setaria . Thus, our results provide evidence of p ‐coumaroylation of AX promoted by SvBAHD05 acyltransferase in the cell wall of the model grass S. viridis . Furthermore, SvBAHD05 is a promising biotechnological target to engineer crops for improved biomass digestibility for biofuels, biorefineries and animal feeding.