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CRISPR‐based assessment of genomic structure in the conserved SQUAMOSA promoter‐binding‐like gene clusters in rice
Author(s) -
Jiang Mengmeng,
He Ying,
Chen Xiaonan,
Zhang Xiaohui,
Guo Yanru,
Yang Sihai,
Huang Ju,
Traw M. Brian
Publication year - 2020
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.15001
Subject(s) - biology , subfunctionalization , synteny , gene , genetics , neofunctionalization , gene family , gene duplication , brachypodium , oryza sativa , genome , promoter , gene expression
We show here conserved paired unit clustering of SPL transcription factors in rice, and document that the pattern is widespread in monocots and dicots. Through comprehensive Cas9 editing of the 19‐member OsSPL family, we find subfunctionalization but not neofunctionalization between paired genes, and widespread fitness‐related defects. We report expression interactions both within and between gene groups. These findings are important to our understanding of gene functional redundancy and retention across evolutionary time.