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LcEIL2/3 are involved in fruitlet abscission via activating genes related to ethylene biosynthesis and cell wall remodeling in litchi
Author(s) -
Ma Xingshuai,
Yuan Ye,
Wu Qian,
Wang Jun,
Li Jianguo,
Zhao Minglei
Publication year - 2020
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14804
Subject(s) - abscission , biology , arabidopsis , ethylene , gene , mutant , microbiology and biotechnology , botany , biochemistry , catalysis
Summary Fruit crops are subject to precocious fruit abscission, during which the phytohormone ethylene (ET) acts as a major positive regulator. However, the molecular basis of ET‐induced fruit abscission remains poorly understood. Here, we show that two ETHYLENE INSENSITIVE 3‐like (EIL) homologs in litchi, LcEIL2 and LcEIL3 , play a role in ET‐activated fruitlet abscission. LcEIL2 / 3 were significantly upregulated in the fruit abscission zone (AZ) during the ET‐induced fruitlet abscission in litchi. The presence of LcEIL2 / 3 in wild‐type Arabidopsis and ein3 eil1 mutants can accelerate the floral organ abscission. Moreover, the electrophoretic mobility shift assay and dual luciferase reporter analysis illustrated that LcEIL2/3 directly interacted with the gene promoters to activate the expression of cell wall remodeling genes LcCEL2 / 8 and LcPG1 / 2 , and ET biosynthetic genes LcACS1 / 4/7 and LcACO2 / 3 . Furthermore, we showed that LcPG1/2 were expressed in the floral abscission zone of Arabidopsis, and constitutive expression of LcPG2 in Arabidopsis promoted the floral organ abscission. In conclusion, we propose that LcEIL2/3 are involved in ET‐induced fruitlet abscission via controlling expression of genes related to ET biosynthesis and cell wall remodeling in litchi.