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Phylogenetic comparison of 5′ splice site determination in central spliceosomal proteins of the U1‐70K gene family, in response to developmental cues and stress conditions
Author(s) -
Chen MoXian,
Zhang KaiLu,
Gao Bei,
Yang JingFang,
Tian Yuan,
Das Debatosh,
Fan Tao,
Dai Lei,
Hao GeFei,
Yang GuangFu,
Zhang Jianhua,
Zhu FuYuan,
Fang YanMing
Publication year - 2020
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14735
Subject(s) - spliceosome , biology , rna splicing , snrnp , small nuclear ribonucleoprotein , intron , genetics , gene , alternative splicing , arabidopsis , splicing factor , ribonucleoprotein , exon , mutant , rna
SUMMARY Intron‐containing genes have the ability to generate multiple transcript isoforms by splicing, thereby greatly expanding the eukaryotic transcriptome and proteome. In eukaryotic cells, precursor mRNA (pre‐mRNA) splicing is performed by a mega‐macromolecular complex defined as a spliceosome. Among its splicing components, U1 small nuclear ribonucleoprotein (U1 snRNP) is the smallest subcomplex involved in early spliceosome assembly and 5′‐splice site recognition. Its central component, named U1‐70K, has been extensively characterized in animals and yeast. Very few investigations on U1‐70K genes have been conducted in plants, however. To this end, we performed a comprehensive study to systematically identify 115 U1‐70K genes from 67 plant species, ranging from algae to angiosperms. Phylogenetic analysis suggested that the expansion of the plant U1‐70K gene family was likely to have been driven by whole‐genome duplications. Subsequent comparisons of gene structures, protein domains, promoter regions and conserved splicing patterns indicated that plant U1‐70K s are likely to preserve their conserved molecular function across plant lineages and play an important functional role in response to environmental stresses. Furthermore, genetic analysis using T‐DNA insertion mutants suggested that Arabidopsis U1‐70K may be involved in response to osmotic stress. Our results provide a general overview of this gene family in Viridiplantae and will act as a reference source for future mechanistic studies on this U1 snRNP‐specific splicing factor.