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Overexpression of the persimmon abscisic acid β‐glucosidase gene ( DkBG1 ) alters fruit ripening in transgenic tomato
Author(s) -
Liang Bin,
Zheng Yu,
Wang Juan,
Zhang Wenbo,
Fu Ying,
Kai Wenbin,
Xu Yandan,
Yuan Bing,
Li Qian,
Leng Ping
Publication year - 2020
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14695
Subject(s) - abscisic acid , ripening , genetically modified tomato , ethylene , biology , gene , transgene , gene expression , horticulture , botany , biochemistry , genetically modified crops , catalysis
Summary β‐Glucosidases (BG) are present in many plant tissues. Among these, abscisic acid (ABA) β‐glucosidases are thought to take part in the adjustment of cellular ABA levels, however the role of ABA‐BG in fruits is still unclear. In this study, through RNA‐seq analysis of persimmon fruit, 10 full‐length DkBG genes were isolated and were all found to be expressed. In particular, DkBG1 was highly expressed in persimmon fruits with a maximum expression 95 days after full bloom (DAFD). We verified that, in vitro , DkBG1 protein can hydrolyze ABA‐glucose ester (ABA‐GE) to release free ABA. Compared with wild‐type, tomato plants that overexpressed DkBG1 significantly upregulated the expression of ABA receptor PYL3/7 genes and showed typical symptoms of ABA hypersensitivity in fruits. DkBG1 overexpression ( DkBG1‐ OE) accelerated fruit ripening onset by 3–4 days by increasing ABA levels at the pre‐breaker stage and induced early ethylene release compared with wild‐type fruits. DkBG1‐ OE altered the expression of ripening regulator NON‐RIPENING ( NOR ) and its target genes; this in turn altered fruit quality traits such as coloration. Our results demonstrated that DkBG1 plays an important role in fruit ripening and quality by adjusting ABA levels via hydrolysis of ABA‐GE.