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Intronic heterochromatin prevents cryptic transcription initiation in Arabidopsis
Author(s) -
Zhou Jincong,
Liu Liangyu,
Li Qin,
Xu Wei,
Li Kuan,
Wang ZhiWei,
Sun Qianwen
Publication year - 2020
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14584
Subject(s) - biology , heterochromatin , genetics , rna polymerase ii , transposable element , transcription (linguistics) , genome , heterochromatin protein 1 , gene , rna , chromatin , gene expression , promoter , linguistics , philosophy
Summary Intronic transposable elements (TEs) comprise a large proportion in eukaryotic genomes, but how they regulate the host genes remains to be explored. Our forward genetic screen disclosed the plant‐specific RNA polymerases IV and V in suppressing intronic TE‐mediated cryptic transcription initiation of a chimeric transcripts at FLC ( FLC TE ). Initiation of FLC TE transcription is blocked by the locally formed intronic heterochromatin, which is directly associated with RNA Pol V to inhibit the entry of RNA Pol II and the occupancy of H3K4 methylation. Genome‐wide Pol II Ser5p native elongation transcription sequencing revealed that a significant number of intronic heterochromatin‐containing genes undergo this mechanism. This study sheds light on deeply understanding the function of intronic heterochromatin on host genes expression in eukaryotic genome.