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Cell wall contributes to the stability of plasma membrane nanodomain organization of Arabidopsis thaliana FLOTILLIN2 and HYPERSENSITIVE INDUCED REACTION1 proteins
Author(s) -
Daněk Michal,
Angelini Jindřiška,
Malínská Kateřina,
Andrejch Jan,
Amlerová Zuzana,
Kocourková Daniela,
Brouzdová Jitka,
Valentová Olga,
Martinec Jan,
Petrášek Jan
Publication year - 2020
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14566
Subject(s) - fluorescence recovery after photobleaching , arabidopsis thaliana , cytoskeleton , biophysics , microbiology and biotechnology , microtubule , extracellular , actin , biology , arabidopsis , chemistry , stalk , confocal microscopy , biochemistry , membrane , cell , mutant , gene , horticulture
Summary Current models of plasma membrane (PM) postulate its organization in various nano‐ and micro‐domains with distinct protein and lipid composition. While metazoan PM nanodomains usually display high lateral mobility, the dynamics of plant nanodomains is often highly spatially restricted. Here we have focused on the determination of the PM distribution in nanodomains for Arabidopsis thaliana flotillin ( At FLOT) and hypersensitive induced reaction proteins ( At HIR), previously shown to be involved in response to extracellular stimuli. Using in vivo laser scanning and spinning disc confocal microscopy in Arabidopsis thaliana we present here their nanodomain localization in various epidermal cell types. Fluorescence recovery after photobleaching (FRAP) and kymographic analysis revealed that PM‐associated At FLOTs contain significantly higher immobile fraction than At HIRs. In addition, much lower immobile fractions have been found in tonoplast pool of At HIR3. Although members of both groups of proteins were spatially restricted in their PM distribution by corrals co‐aligning with microtubules (MTs), pharmacological treatments showed no or very low role of actin and microtubular cytoskeleton for clustering of At FLOT and At HIR into nanodomains. Finally, pharmacological alteration of cell wall (CW) synthesis and structure resulted in changes in lateral mobility of At FLOT2 and At HIR1. Accordingly, partial enzymatic CW removal increased the overall dynamics as well as individual nanodomain mobility of these two proteins. Such structural links to CW could play an important role in their correct positioning during PM communication with extracellular environment.