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Auxin signaling modulates LATERAL ROOT PRIMORDIUM 1 ( LRP 1 ) expression during lateral root development in Arabidopsis
Author(s) -
Singh Sharmila,
Yadav Sandeep,
Singh Alka,
Mahima Mahima,
Singh Archita,
Gautam Vibhav,
Sarkar Ananda K.
Publication year - 2020
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14520
Subject(s) - auxin , primordium , lateral root , microbiology and biotechnology , lrp1 , biology , arabidopsis , mutant , gene , genetics , biochemistry , lipoprotein , ldl receptor , cholesterol
Summary Auxin signaling mediated by various auxin/indole‐3‐acetic acid (Aux/ IAA s) and AUXIN RESPONSE FACTOR s ( ARF s) regulate lateral root ( LR ) development by controlling the expression of downstream genes. LATERAL ROOT PRIMORDIUM 1 ( LRP 1 ), a member of the SHORT INTERNODES / STYLISH ( SHI / STY ) family, was identified as an auxin‐inducible gene. The precise developmental role and molecular regulation of LRP 1 in root development remain to be understood. Here we show that LRP 1 is expressed in all stages of LR development, besides the primary root. The expression of LRP 1 is regulated by histone deacetylation in an auxin‐dependent manner. Our genetic interaction studies showed that LRP 1 acts downstream of auxin responsive Aux/ IAA s‐ ARF s modules during LR development. We showed that auxin‐mediated induction of LRP 1 is lost in emerging LRs of slr‐1 and arf7arf19 mutants roots. NPA treatment studies showed that LRP 1 acts after LR founder cell specification and asymmetric division during LR development. Overexpression of LRP 1 ( LRP 1 OE ) showed an increased number of LR primordia (LRP) at stages I, IV and V, resulting in reduced emerged LR density, which suggests that it is involved in LRP development. Interestingly, LRP 1‐induced expression of YUC 4 , which is involved in auxin biosynthesis, contributes to the increased accumulation of endogenous auxin in LRP 1 OE roots. LRP 1 interacts with SHI , STY 1, SRS 3, SRS6 and SRS 7 proteins of the SHI / STY family, indicating their possible redundant role during root development. Our results suggested that auxin and histone deacetylation affect LRP 1 expression and it acts downstream of LR forming auxin response modules to negatively regulate LRP development by modulating auxin homeostasis in Arabidopsis thaliana .