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DELAY OF GERMINATION 1‐ LIKE 4 acts as an inducer of seed reserve accumulation
Author(s) -
Sall Khadidiatou,
Dekkers Bas J. W.,
ogaki Mariko,
Katsuragawa Yoshihiko,
Koyari Ryosuke,
Hendrix David,
Willems Leo A. J.,
Bentsink Leónie,
ogaki Hiroyuki
Publication year - 2019
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14485
Subject(s) - abscisic acid , biology , dormancy , seed dormancy , germination , mutant , gene , microbiology and biotechnology , biochemistry , botany
Summary More than 70% of global food supply depends on seeds. The major seed reserves, such as proteins, lipids, and polysaccharides, are produced during seed maturation. Here, we report that DELAY OF GERMINATION 1‐ LIKE 4 ( DOGL 4) is a major inducer of reserve accumulation during seed maturation. The DOGL family proteins are plant‐specific proteins of largely unknown biochemical function. DOGL 4 shares only limited homology in amino acid sequence with DOG 1, a major regulator of seed dormancy. DOGL 4 was identified as one of the outstanding abscisic acid ( ABA )‐induced genes in our RNA sequencing analysis, whereas DOG 1 was not induced by ABA . Induction of DOGL 4 caused the expression of 70 seed maturation‐specific genes, even in germinating seeds, including the major seed reserves ALBUMIN , CRUCIFERIN and OLEOSIN . Although DOG 1 affects the expression of many seed maturation genes, the major seed reserve genes induced by DOGL 4 are not altered by the dog1 mutation. Furthermore, the reduced dormancy and longevity phenotypes observed in the dog1 seeds were not observed in the dogl4 mutants, suggesting that these two genes have limited functional overlap. Taken together, these results suggest that DOGL 4 is a central factor mediating reserve accumulation in seeds, and that the two DOG 1 family proteins have diverged over the course of evolution into independent regulators of seed maturation, but retain some overlapping function.