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Systematic identification of long noncoding RNA s expressed during light‐induced anthocyanin accumulation in apple fruit
Author(s) -
Yang Tuo,
Ma Huaying,
Zhang Jie,
Wu Ting,
Song Tingting,
Tian Ji,
Yao Yuncong
Publication year - 2019
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14470
Subject(s) - anthocyanin , rna , biology , rna silencing , gene expression , malus , gene silencing , biochemistry , microbiology and biotechnology , botany , gene , rna interference
Summary Anthocyanin pigments contribute to the red color of apple ( Malus × domestica ) fruit and have a major influence on their ornamental, dietary and market value. In this study, we investigated the potential role of long noncoding RNA s (lnc RNA s) in anthocyanin biosynthesis. RNA ‐seq analysis of apple peels from the ‘Red Fuji’ cultivar during light‐induced rapid anthocyanin accumulation revealed 5297 putative lnc RNA s. Differential expression analysis further showed that lnc RNA s were induced during light treatment and were involved in photosynthesis. Using the mi RNA −lnc RNA − mRNA network and endogenous target mimic ( eTM ) analysis, we predicted that two differentially expressed lnc RNA s, MLNC 3.2 and MLNC 4.6, were potential eTM s for mi RNA 156a and promoted the expression of the SPL 2‐like and SPL 33 transcription factors. Transient expression in apple fruit and stable transformation of apple callus showed that overexpression of the eTM s and SPL s promoted anthocyanin accumulation, with the opposite results in eTM and SPL ‐silenced fruit. Silencing or overexpressing of miR156a also affected the expression of the identified eTM s and SPL s. These results indicated that MLNC 3.2 and MLNC 4.6 function as eTM s for miR156a and prevent cleavage of SPL 2‐like and SPL 33 by miR156a during light‐induced anthocyanin biosynthesis. Our study provides fundamental insights into lnc RNA involvement in the anthocyanin biosynthetic pathway in apple fruit.