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GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca 2+ signaling pathways in transgenic soybean
Author(s) -
Wei Wei,
Liang DaWei,
Bian XiaoHua,
Shen Ming,
Xiao JianHui,
Zhang WanKe,
Ma Biao,
Lin Qing,
Lv Jian,
Chen Xi,
Chen ShouYi,
Zhang JinSong
Publication year - 2019
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14449
Subject(s) - wrky protein domain , abscisic acid , biology , drought tolerance , gene , abiotic stress , arabidopsis , microbiology and biotechnology , genetically modified crops , transgene , signal transduction , gene expression , transcription factor , genetics , botany , mutant
Summary WRKY transcription factors play important roles in response to various abiotic stresses. Previous study have proved that soybean GmWRKY54 can improve stress tolerance in transgenic Arabidopsis. Here, we generated soybean transgenic plants and further investigated roles and biological mechanisms of GmWRKY54 in response to drought stress. We demonstrated that expression of GmWRKY54 , driven by either a constitutive promoter (p Cm ) or a drought‐induced promoter ( RD29a ), confers drought tolerance. GmWRKY54 is a transcriptional activator and affects a large number of stress‐related genes as revealed by RNA sequencing. Gene ontology (GO) enrichment and co‐expression network analysis, together with measurement of physiological parameters, supported the idea that GmWRKY54 enhances stomatal closure to reduce water loss, and therefore confers drought tolerance in soybean. GmWRKY54 directly binds to the promoter regions of genes including PYL8 , SRK2A , CIPK11 and CPK3 and activates them. Therefore GmWRKY54 achieves its function through abscisic acid (ABA) and Ca 2+ signaling pathways. It is valuable that GmWRKY54 activates an ABA receptor and an SnRK2 kinase in the upstream position, unlike other WRKY proteins that regulate downstream genes in the ABA pathway. Our study revealed the role of GmWRKY54 in drought tolerance and further manipulation of this gene should improve growth and production in soybean and other legumes/crops under unfavorable conditions.