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CRD 1, an Xpo1 domain protein, regulates mi RNA accumulation and crown root development in rice
Author(s) -
Zhu Jianshu,
Li Yong,
Lin Jian,
Wu Yunrong,
Guo Huaxing,
Shao Yanlin,
Wang Fei,
Wang Xiaofei,
Mo Xiaorong,
Zheng Shaojian,
Yu Hao,
Mao Chuanzao
Publication year - 2019
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14445
Subject(s) - mutant , complementation , microbiology and biotechnology , biology , biochemistry , gene
Summary Crown root (CR) is the main component of the fibrous root system in cereal crops, but the molecular mechanism underlying CR development is still unclear. Here, we isolated the crown root defect 1 ( crd1 ) mutant from ethyl methane sulfonate‐mutated mutant library, which significantly inhibited CR development. The CRD 1 was identified through genome resequencing and complementation analysis, which encodes an Xpo1 domain protein: the rice ortholog of Arabidopsis HASTY ( HST ) and human exportin‐5 ( XPO 5). CRD 1 is ubiquitously expressed, with the highest expression levels in the CR primordium at the stem base. CRD 1 is a nucleocytoplasmic protein. The crd1 mutant contains significantly reduced mi RNA levels in the cytoplasm and nucleus, suggesting that CRD 1 is essential for maintaining normal mi RNA levels in plant cells. The altered CR phenotype of crd1 was simulated by target mimicry of miR156, suggesting that this defect is due to the disruption of miR156 regulatory pathways. Our analysis of CRD 1, the HST ortholog identified in monocots, expands our understanding of the molecular mechanisms underlying mi RNA level and CR development.