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Boron demanding tissues of Brassica napus express specific sets of functional Nodulin26‐like Intrinsic Proteins and BOR 1 transporters
Author(s) -
Diehn Till Arvid,
Bienert Manuela Désirée,
Pommerrenig Benjamin,
Liu Zhaojun,
Spitzer Christoph,
Bernhardt Nadine,
Fuge Jacqueline,
Bieber Annett,
Richet Nicolas,
Chaumont François,
Bienert Gerd Patrick
Publication year - 2019
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14428
Subject(s) - nip , transporter , gene isoform , biology , yeast , heterologous expression , biochemistry , microbiology and biotechnology , chemistry , gene , recombinant dna , computer science , programming language
Summary The sophisticated uptake and translocation regulation of the essential element boron (B) in plants is ensured by two transmembrane transporter families: the Nodulin26‐like Intrinsic Protein ( NIP ) and BOR transporter family. Though the agriculturally important crop Brassica napus is highly sensitive to B deficiency, and NIP s and BOR s have been suggested to be responsible for B efficiency in this species, functional information of these transporter subfamilies is extremely rare. Here, we molecularly characterized the NIP and BOR 1 transporter family in the European winter‐type cv. Darmor‐ PBY 018 . Our transport assays in the heterologous oocyte and yeast expression systems as well as in growth complementation assays in planta demonstrated B transport activity of NIP 5, NIP 6, NIP 7 and BOR 1 isoforms. Moreover, we provided functional and quantitative evidence that also members of the NIP 2, NIP 3 and NIP 4 groups facilitate the transport of B. A detailed B‐ and tissue‐dependent B‐transporter expression map was generated by quantitative polymerase chain reaction. We showed that NIP 5 isoforms are highly upregulated under B‐deficient conditions in roots, but also in shoot tissues. Moreover, we detected transcripts of several B‐permeable NIP s from various groups in floral tissues that contribute to the B distribution within the highly B deficiency‐sensitive flowers.