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The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses
Author(s) -
TéllezRobledo Barbara,
Manzano Concepcion,
Saez Angela,
NavarroNeila Sara,
SilvaNavas Javier,
Lorenzo Laura,
GonzálezGarcía MaryPaz,
Toribio René,
Hunt Arthur G.,
Baigorri Roberto,
Casimiro Ilda,
Brady Siobhan M.,
Castellano M. Mar,
Pozo J. Carlos
Publication year - 2019
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14416
Subject(s) - polyadenylation , abiotic component , biology , abiotic stress , untranslated region , microbiology and biotechnology , gene , genetics , gene expression , messenger rna , ecology
Summary Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1 , a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5′ untranslated regions (5′‐UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non‐canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1‐2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1‐mediated alternative polyadenylation is important for plant development and stress responses.