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Overexpression of trans ‐Golgi network t‐ SNARE s rescues vacuolar trafficking and TGN morphology defects in a putative tethering factor mutant
Author(s) -
Yang Xiaochen,
Liao ChingYi,
Tang Jie,
Bassham Diane C.
Publication year - 2019
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14353
Subject(s) - microbiology and biotechnology , golgi apparatus , vacuole , mutant , vacuolar protein sorting , cytoplasm , biology , tethering , transport protein , chemistry , endoplasmic reticulum , biochemistry , gene
Summary The trans ‐Golgi network ( TGN ) is a major site for sorting of cargo to either the vacuole or apoplast. The TGN ‐localized coiled‐coil protein TNO 1 is a putative tethering factor that interacts with the TGN t‐ SNARE SYP 41 and is required for correct localization of the SYP 61 t‐ SNARE . An Arabidopsis thaliana tno1 mutant is hypersensitive to salt stress and partially mislocalizes vacuolar proteins to the apoplast, indicating a role in vacuolar trafficking. Here, we show that overexpression of SYP 41 or SYP 61 significantly increases SYP 41– SYP 61 complex formation in a tno1 mutant, and rescues the salt sensitivity and defective vacuolar trafficking of the tno1 mutant. The TGN is disrupted and vesicle budding from Golgi cisternae is reduced in the tno1 mutant, and these defects are also rescued by overexpression of SYP 41 or SYP 61. Our results suggest that the trafficking and Golgi morphology defects caused by loss of TNO 1 can be rescued by increasing SYP 41– SYP 61 t‐ SNARE complex formation, implicating TNO 1 as a tethering factor mediating efficient vesicle fusion at the TGN .