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At BUD 13 affects pre‐ mRNA splicing and is essential for embryo development in Arabidopsis
Author(s) -
Xiong Feng,
Ren JingJing,
Yu Qin,
Wang YuYi,
Kong LanJing,
Otegui Marisa S.,
Wang XiuLing
Publication year - 2019
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14268
Subject(s) - biology , arabidopsis , intron , rna splicing , gene , genetics , microbiology and biotechnology , embryo , rna binding protein , mutant , messenger rna , rna
Summary Pre‐ mRNA splicing is an important step for gene expression regulation. Yeast Bud13p (bud‐site selection protein 13) regulates the budding pattern and pre‐ mRNA splicing in yeast cells; however, no Bud13p homologs have been identified in plants. Here, we isolated two mutants that carry T‐ DNA insertions at the At1g31870 locus and shows early embryo lethality and seed abortion. At1g31870 encodes an Arabidopsis homolog of yeast Bud13p, At BUD 13. Although At BUD 13 homologs are widely distributed in eukaryotic organisms, phylogenetic analysis revealed that their protein domain organization is more complex in multicellular species. At BUD 13 is expressed throughout plant development including embryogenesis and At BUD 13 proteins is localized in the nucleus in Arabidopsis. RNA ‐seq analysis revealed that At BUD 13 mutation predominantly results in the intron retention, especially for shorter introns (≤100 bases). Within this group of genes, we identified 52 genes involved in embryogenesis, out of which 22 are involved in nucleic acid metabolism. Our results demonstrate that At BUD 13 plays critical roles in early embryo development by effecting pre‐ mRNA splicing.