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Jasmonic acid‐inducible TSA 1 facilitates ER body formation
Author(s) -
Geem Kyoung Rok,
Kim Dae Heon,
Lee Dong Wook,
Kwon Yun,
Lee Junho,
Kim Jeong Hee,
Hwang Inhwan
Publication year - 2019
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14112
Subject(s) - nicotiana benthamiana , endoplasmic reticulum , unfolded protein response , microbiology and biotechnology , arabidopsis thaliana , biogenesis , methyl jasmonate , chemistry , ectopic expression , arabidopsis , biology , mutant , biochemistry , gene
Summary Members of the Brassicales contain an organelle, the endoplasmic reticulum ( ER ) body, which is derived from the ER . Recent studies have shed light on the biogenesis of the ER body and its physiological role in plants. However, formation of the ER body and its physiological role are not fully understood. Here, we investigated the physiological role of TSK ‐associating protein 1 ( TSA 1), a close homolog of NAI 2 that is involved in ER body formation, and provide evidence that it is involved in ER body biogenesis under wound‐related stress conditions. TSA 1 is N‐glycosylated and localizes to the ER body as a luminal protein. TSA 1 was highly induced by the plant hormone, methyl jasmonate (Me JA ). Ectopic expression of TSA 1: GFP induced ER body formation in root tissues of transgenic Arabidopsis thaliana and in leaf tissues of Nicotiana benthamiana . TSA 1 and NAI 2 formed a heterocomplex and showed an additive effect on ER body formation in N. benthamiana . Me JA treatment induced ER body formation in leaf tissues of nai2 and tsa1 plants, but not nai2/tsa1 double‐mutant plants. However, constitutive ER body formation was altered in young seedlings of nai2 plants but not tsa1 plants. Based on these results, we propose that TSA 1 plays a critical role in Me JA ‐induced ER body formation in plants.