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Isoflavonoid‐specific prenyltransferase gene family in soybean: GmPT01, a pterocarpan 2‐dimethylallyltransferase involved in glyceollin biosynthesis
Author(s) -
Sukumaran Arjun,
McDowell Tim,
Chen Ling,
Renaud Justin,
Dhaubhadel Sangeeta
Publication year - 2018
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14083
Subject(s) - isoflavonoid , phytoalexin , phytophthora sojae , prenyltransferase , biology , elicitor , gene , gene expression , biochemistry , biosynthesis , flavonoid , antioxidant , resveratrol
Summary Phytoalexin glyceollins are soybean‐specific antimicrobial compounds that are derived from the isoflavonoid pathway. They are synthesized by soybean in response to extrinsic stress such as pathogen attack or injury, thereby conferring partial resistance if synthesized rapidly at the site of infection and at the required concentration. Soybean produces multiple forms of glyceollins that result from the differential prenylation reaction catalyzed by prenyltransferases ( PT s) on either the C‐2 or C‐4 carbon of a pterocarpan glycinol. The soybean genome contains 77 PT ‐encoding genes (Gm PT s) where at least 11 are (iso)flavonoid‐specific. Transcript accumulation of five candidates Gm PT s was increased in response to Phytophthora sojae infection, suggesting their role in phytoalexin synthesis. The induced Gm PT s localize to plastids and display tissue‐specific expression. We have in this study identified two additional Gm PT s: an isoflavone dimethylallyltransferase 3 ( IDT 3); and a glycinol 2‐dimethylallyl transferase Gm PT 01. Gm PT 01 prenylates (−)‐glycinol at the C‐2 position, localizes in the plastid, and exhibits root‐specific gene expression. Furthermore, its expression is induced rapidly in response to stress, and is associated with a quantitative trait loci linked with resistance to P. sojae . Based on these results, we conclude that Gm PT 01 are possibly one of the loci involved in conferring partial resistance against stem and root rot disease in soybean.