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Transcriptomics analyses reveal the molecular roadmap and long non‐coding RNA landscape of sperm cell lineage development
Author(s) -
Liu Lingtong,
Lu Yunlong,
Wei Liqin,
Yu Hua,
Cao Yinghao,
Li Yan,
Yang Ning,
Song Yunyun,
Liang Chengzhi,
Wang Tai
Publication year - 2018
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14041
Subject(s) - biology , lineage (genetic) , transcriptome , gene , somatic cell , microbiology and biotechnology , epigenetics , genetics , gene expression
Summary Sperm cell ( SC ) lineage development from the haploid microspore to SC s represents a unique biological process in which the microspore generates a larger vegetative cell ( VC ) and a smaller generative cell ( GC ) enclosed in the VC , then the GC further develops to functionally specified SC s in the VC for double fertilization. Understanding the mechanisms of SC lineage development remains a critical goal in plant biology. We isolated individual cells of the three cell types, and characterized the genome‐wide atlas of long non‐coding (lnc) RNA s and mRNA s of haploid SC lineage cells. Sperm cell lineage development involves global repression of genes for pluripotency, somatic development and metabolism following asymmetric microspore division and coordinated upregulation of GC / SC preferential genes. This process is accompanied by progressive loss of the active marks H3K4me3 and H3K9ac, and accumulation of the repressive methylation mark H3K9. The SC lineage has a higher ratio of lnc RNA s to mRNA s and preferentially expresses a larger percentage of lnc RNA s than does the non‐ SC lineage. A co‐expression network showed that the largest set of lnc RNA s in these nodes, with more than 100 links, are GC ‐preferential, and a small proportion of lnc RNA s co‐express with their neighboring genes. Single molecular fluorescence in situ hybridization showed that several candidate genes may be markers distinguishing the three cell types of the SC lineage. Our findings reveal the molecular programming and potential roles of lnc RNA s in SC lineage development.