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The potato transcription factor Stb ZIP 61 regulates dynamic biosynthesis of salicylic acid in defense against Phytophthora infestans infection
Author(s) -
Zhou XinTong,
Jia LiJia,
Wang HaiYun,
Zhao Pan,
Wang WenYan,
Liu Ning,
Song ShuangWei,
Wu Yao,
Su Lei,
Zhang Jie,
Zhong NaiQin,
Xia GuiXian
Publication year - 2018
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.14010
Subject(s) - phytophthora infestans , biology , transcription factor , salicylic acid , jasmonic acid , microbiology and biotechnology , biochemistry , gene
Summary Salicylic acid ( SA ) signalling plays an essential role in plant innate immunity. In this study, we identified a component in the SA signaling pathway in potato ( Solanum tuberosum ), the transcription factor Stb ZIP 61 , and characterized its function in defence against Phytophthora infestans . Expression of Stb ZIP 61 was induced upon P . infestans infection and following exposure to the defense signaling hormones SA , ethylene and jasmonic acid. Overexpression of Stb ZIP 61 increased the tolerance of potato plants to P . infestans while RNA interference ( RNA i) increased susceptibility. Yeast two‐hybrid and pull down experiments revealed that Stb ZIP 61 could interact with an NPR 3‐like protein (St NPR 3L) that inhibited its DNA ‐binding and transcriptional activation activities. Moreover, St NPR 3L interacted with Stb ZIP 61 in an SA ‐dependent manner. Among candidate genes involved in SA ‐regulated defense responses, Stb ZIP 61 had a significant impact on expression of St ICS 1 , which encodes a key enzyme for SA biosynthesis. St ICS 1 transcription was induced upon P . infestans infection and this responsive expression to the pathogen was reduced in Stb ZIP 61 RNA i plants. Accordingly, St ICS 1 expression was remarkably enhanced in Stb ZIP 61 ‐overexpressing plants. Together, our data demonstrate that Stb ZIP 61 functions in concert with St NPR 3L to regulate the temporal activation of SA biosynthesis, which contributes to SA ‐mediated immunity against P . infestans infection in potato.