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LFR is functionally associated with AS 2 to mediate leaf development in Arabidopsis
Author(s) -
Lin Xiaowei,
Gu Dandan,
Zhao Hongtao,
Peng Yue,
Zhang Guofang,
Yuan Tingting,
Li Mengge,
Wang Zhijuan,
Wang Xiutang,
Cui Sujuan
Publication year - 2018
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13973
Subject(s) - biology , arabidopsis , mutant , chromatin , petiole (insect anatomy) , arabidopsis thaliana , genetics , microbiology and biotechnology , psychological repression , enhancer , transcription factor , gene , botany , gene expression , hymenoptera
Summary Leaves are essential organs for plants. We previously identified a functional gene possibly encoding a component of the SWI / SNF complex named Leaf and Flower Related ( LFR ) in Arabidopsis thaliana . Loss‐of‐function mutants of LFR displayed obvious defects in leaf morphogenesis, indicating its vital role in leaf development. Here an allelic null mutant of ASYMMETRIC LEAVES 2 ( AS 2 ), as2‐6 , was isolated as an enhancer of lfr‐1 in petiole length, vasculature pattern and leaf margin development. The lfr as 2 double‐mutants showed enhanced ectopic expression of BREVIPEDICELLUS ( BP ) compared with each of the single‐mutants, which is consistent with their synergistic genetic enhancement in multiple BP ‐dependent development processes. Moreover, LFR and several putative subunits of the SWI / SNF complex interacted physically with AS 2. LFR associated with BP chromatin in an AS 1– AS 2‐dependent manner to promote the nucleosome occupancy for appropriate BP repression in leaves. Taken together, our findings reveal that LFR and the SWI / SNF complex play roles in leaf development at least partly by repressing BP transcription as interacting factors of AS 2, which expounds our understanding of BP repression at the chromatin structure level in leaf development.