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LCM‐seq reveals the crucial role of LsSOC1 in heat‐promoted bolting of lettuce ( Lactuca sativa L.)
Author(s) -
Chen Zijing,
Zhao Wensheng,
Ge Danfeng,
Han Yingyan,
Ning Kang,
Luo Chen,
Wang Shenglin,
Liu Renyi,
Zhang Xiaolan,
Wang Qian
Publication year - 2018
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13968
Subject(s) - bolting , lactuca , biology , mutant , transcriptome , botany , gene , genetics , gene expression
Summary Lettuce ( Lactuca sativa L.) is one of the most economically important vegetables. The floral transition in lettuce is accelerated under high temperatures, which can significantly decrease yields. However, the molecular mechanism underlying the floral transition in lettuce is poorly known. Using laser capture microdissection coupled with RNA sequencing, we isolated shoot apical meristem cells from the bolting‐sensitive lettuce line S39 at four critical stages of development. Subsequently, we screened specifically for the flowering‐related gene LsSOC1 during the floral transition through comparative transcriptomic analysis. Molecular biology, developmental biology, and biochemical tools were combined to investigate the biological function of Ls SOC 1 in lettuce. Ls SOC 1 knockdown by RNA interference resulted in a significant delay in the timing of bolting and insensitivity to high temperature, which indicated that Ls SOC 1 functions as an activator during heat‐promoted bolting in lettuce. We determined that two heat shock transcription factors, HsfA1e and HsfA4c , bound to the promoter of Ls SOC 1 to confirm that Ls SOC 1 played an important role in heat‐promoted bolting. This study indicates that Ls SOC 1 plays a crucial role in the heat‐promoted bolting process in lettuce. Further investigation of Ls SOC 1 may be useful for clarification of the bolting mechanism in lettuce.