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Intact Arabidopsis RPB 1 functions in stem cell niches maintenance and cell cycling control
Author(s) -
Zhang QianQian,
Li Ying,
Fu ZhaoYing,
liu XunBiao,
Yuan Kai,
Fang Ying,
Liu Yan,
Li Gang,
Zhang XianSheng,
Chong Kang,
Ge Lei
Publication year - 2018
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13939
Subject(s) - biology , meristem , ctd , arabidopsis , microbiology and biotechnology , gene , cell cycle , stem cell , transcription (linguistics) , transcription factor , botany , genetics , mutant , linguistics , oceanography , philosophy , geology
Summary Plant meristem activity depends on accurate execution of transcriptional networks required for establishing optimum functioning of stem cell niches. An Arabidopsis mutant card1‐1 ( c onstitutive a uxin r esponse withD R 5 : GFP ) that encodes a truncated RPB 1 ( R NA P olymerase II 's largest su b unit) with shortened C‐terminal domain ( CTD ) was identified. Phosphorylation of the CTD repeats of RPB 1 is coupled to transcription in eukaryotes. Here we uncover that the truncated CTD of RPB 1 disturbed cell cycling and enlarged the size of shoot and root meristem. The defects in patterning of root stem cell niche in card1‐1 indicates that intact CTD of RPB 1 is necessary for fine‐tuning the specific expression of genes responsible for cell‐fate determination. The gene‐edited plants with different CTD length of RPB 1, created by CRISPR ‐ CAS 9 technology, confirmed that both the full length and the DK ‐rich tail of RPB 1's CTD play roles in the accurate transcription of CYCB 1;1 encoding a cell‐cycle marker protein in root meristem and hence participate in maintaining root meristem size. Our experiment proves that the intact RPB 1 CTD is necessary for stem cell niche maintenance, which is mediated by transcriptional regulation of cell cycling genes.