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Cytidinediphosphate‐diacylglycerol synthase 5 is required for phospholipid homeostasis and is negatively involved in hyperosmotic stress tolerance
Author(s) -
Hong Yue,
Yuan Shu,
Sun Linxiao,
Wang Xuemin,
Hong Yueyun
Publication year - 2018
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13916
Subject(s) - phosphatidic acid , phospholipase d , phosphatidylinositol , diacylglycerol kinase , osmotic concentration , osmotic shock , mutant , biology , microbiology and biotechnology , biochemistry , phospholipid , thylakoid , atp synthase , chemistry , signal transduction , enzyme , protein kinase c , chloroplast , membrane , gene
Summary Cytidinediphosphate diacylglycerol synthase ( CDS ) uses phosphatidic acid ( PA ) and cytidinetriphosphate to produce cytidinediphosphate‐diacylglycerol, an intermediate for phosphatidylglycerol ( PG ) and phosphatidylinositol ( PI ) synthesis. This study shows that CDS 5, one of the five CDS s of the Oryza sativa (rice) genome, has multifaceted effects on plant growth and stress responses. The loss of CDS 5 resulted in a decrease in PG and PI levels, defective thylakoid membranes, pale leaves in seedlings and growth retardation. In addition, the loss of CDS 5 led to an elevated PA level and enhanced hyperosmotic tolerance. The inhibition of phospholipase D ( PLD )‐derived PA formation in cds5 restored the hyperosmotic stress tolerance of the mutant phenotype to that of the wild type, suggesting that CDS 5 functions as a suppressor in PLD ‐derived PA signaling and negatively affects hyperosmotic stress tolerance.