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Phosphoproteomic profiling reveals ABA ‐responsive phosphosignaling pathways in Physcomitrella patens
Author(s) -
Amagai Anna,
Honda Yoshimasa,
Ishikawa Shinnosuke,
Hara Yurie,
Kuwamura Mayuri,
Shinozawa Akihisa,
Sugiyama Naoyuki,
Ishihama Yasushi,
Takezawa Daisuke,
Sakata Yoichi,
Shinozaki Kazuo,
Umezawa Taishi
Publication year - 2018
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13891
Subject(s) - physcomitrella patens , abscisic acid , biology , bryopsida , mutant , phosphorylation , phosphatase , regulator , microbiology and biotechnology , biochemistry , gene
Summary Abscisic acid (ABA) and its signaling system are important for land plants to survive in terrestrial conditions. Here, we took a phosphoproteomic approach to elucidate the ABA signaling network in Physcomitrella patens , a model species of basal land plants. Our phosphoproteomic analysis detected 4630 phosphopeptides from wild‐type P . patens and two ABA‐responsive mutants, a disruptant of group‐A type‐2C protein phosphatase (PP2C; ppabi1a/b ) and AR7, a defective mutant in ARK, identified as an upstream regulator of SnRK2. Quantitative analysis detected 143 ABA‐responsive phosphopeptides in P . patens . The analysis indicated that SnRK2‐mediated phosphorylation and target motifs were partially conserved in bryophytes. Our data demonstrate that the PpSnRK2B and AREB/ABF‐type transcription factors are phosphorylated in vivo in response to ABA under the control of ARK. On the other hand, our data also revealed the following: (i) the entire ABA‐responsive phosphoproteome in P . patens is quite diverse; (ii) P . patens PP2C affects additional pathways other than the known ABA signaling pathway; and (iii) ARK is mainly involved in ABA signaling. Taken together, we propose that the core ABA signaling pathway is essential in all land plants; however, some ABA‐responsive phosphosignaling uniquely developed in bryophytes during the evolutionary process.