Premium
Natural variation in the promoter of rice calcineurin B‐like protein10 (Os CBL 10) affects flooding tolerance during seed germination among rice subspecies
Author(s) -
Ye NengHui,
Wang FengZhu,
Shi Lu,
Chen MoXian,
Cao YunYing,
Zhu FuYuan,
Wu YiZhen,
Xie LiJuan,
Liu TieYuan,
Su ZeZhuo,
Xiao Shi,
Zhang Hao,
Yang Jianchang,
Gu HaiYong,
Hou XuanXuan,
Hu QiJuan,
Yi HuiJuan,
Zhu ChangXiang,
Zhang Jianhua,
Liu YingGao
Publication year - 2018
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13881
Subject(s) - oryza sativa , cultivar , flooding (psychology) , germination , biology , ecotype , japonica , oryza , botany , horticulture , gene , genetics , psychology , psychotherapist
Summary Rice ( Oryza sativa L.) has two ecotypes, upland and lowland rice, that have been observed to show different tolerance levels under flooding stress. In this study, two rice cultivars, upland (Up221, flooding‐intolerant) and lowland (Low88, flooding‐tolerant), were initially used to study their molecular mechanisms in response to flooding germination. We observed that variations in the Os CBL 10 promoter sequences in these two cultivars might contribute to this divergence in flooding tolerance. Further analysis using another eight rice cultivars revealed that the Os CBL 10 promoter could be classified as either a flooding‐tolerant type (T‐type) or a flooding‐intolerant type (I‐type). The Os CBL 10 T‐type promoter only existed in japonica lowland cultivars, whereas the Os CBL 10 I‐type promoter existed in japonica upland, indica upland and indica lowland cultivars. Flooding‐tolerant rice cultivars containing the Os CBL 10 T‐type promoter have shown lower Ca 2+ flow and higher α‐amylase activities in comparison to those in flooding‐intolerant cultivars. Furthermore, the Os CBL 10 overexpression lines were sensitive to both flooding and hypoxic treatments during rice germination with enhanced Ca 2+ flow in comparison to wild‐type. Subsequent findings also indicate that Os CBL 10 may affect Os CIPK 15 protein abundance and its downstream pathways. In summary, our results suggest that the adaptation to flooding stress during rice germination is associated with two different Os CBL 10 promoters, which in turn affect Os CBL 10 expression in different cultivars and negatively affect Os CIPK 15 protein accumulation and its downstream cascade.